NAD+ Guide

NAD+ Guide:

Author: Jeff Robinson B.S. Exercise Physiology Human Performance – Project
Biohacked LLC

The following is for INFORMATIONAL Purposes only, this is NOT MEDICAL
advice, I AM NOT a medical professional, please consult with your physician
before making any changes to your medical treatment plan.

Peptides purchased DIRECT ONLINE are sold for research purposes only, the following information is for REGISTERED researchers.

Important Reminder: Peptides purchased direct online do not come with instructions, they are sold for RESEARCH PURPOSES ONLY – NOT FOR HUMAN CONSUMPTION.

They come in lyophilized powder form and need to be reconstituted at
the proper ratio. To research peptides purchased direct SELF EDUCATION is a MUST.

What is NAD+:

NAD⁺ (Nicotinamide Adenine Dinucleotide) is a coenzyme — a helper molecule that enables other enzymes to function. It’s found in every cell of your body and is essential for life. It exists in two forms:

• NAD⁺ – the oxidized form (accepts electrons)
• NADH – the reduced form (donates electrons)

This dynamic redox pair is crucial for energy transfer and countless cellular
processes.

Potential Benefits from NAD+:

1. Cognitive Function & Brain Health

• Supports mitochondrial function in neurons, improving mental energy.
• Activates sirtuins (SIRT1 & SIRT3) that protect against neurodegeneration.
• Enhances neuronal resilience under stress and may slow progression in:
  • Alzheimer’s disease
  • Parkinson’s disease
  • Age-related memory loss
• May support focus, clarity, and mood.

2. Energy & Metabolic Health

• Crucial for converting food into ATP, the cell’s energy currency.
• Helps regulate glucose and fat metabolism.
• Supports insulin sensitivity and metabolic flexibility.
• Often associated with increased stamina and reduced fatigue.

3. DNA Repair & Cellular Health

• NAD⁺ activates PARP enzymes, which fix DNA strand breaks.
• Helps maintain genomic stability, a core factor in preventing cancer and aging.
• Promotes cell survival under oxidative stress or inflammation.

4. Slows Aging Processes

• Boosts sirtuin activity, which regulates:
  • Gene expression
  • Inflammation
  • Mitochondrial function
  • Telomere maintenance
• Declining NAD⁺ is linked with the onset of age-related diseases.
• Restoration is a central focus in longevity research.

5. Immune System Regulation

• NAD⁺ helps immune cells respond to infection and inflammation.
• Low NAD⁺ is tied to chronic inflammation and “inflammaging.”
• Supports innate immunity and adaptive immune responses.

6. Physical Performance & Recovery

• Improves muscle function and mitochondrial density.
• May enhance exercise endurance and reduce post-workout fatigue.
• Supports tissue repair, especially in muscle and nerve cells.

7. Cardiovascular Support

• Improves endothelial function (inner lining of blood vessels).
• Reduces arterial stiffness and may help lower blood pressure.
• Enhances mitochondrial energy metabolism in heart cells.

8. Potential Therapeutic Uses (Still Under Study)

• Neurodegenerative diseases (Alzheimer’s, ALS, Parkinson’s)
• Metabolic disorders (diabetes, fatty liver)
• Chronic fatigue syndrome
• Post-viral syndromes (e.g., post-COVID)
• Addiction recovery (used in some IV NAD+ clinics)

Brain – Neurorestoration – Addiction/Drug Recovery
Benefits of NAD+:

NEURORESTORATION MECHANISMS

1. Mitochondrial Biogenesis & Function

• Why it matters: Neurons are energy-hungry; mitochondrial dysfunction is linked to neurodegeneration.
• NAD+ role: Activates PGC-1α via SIRT1 → ↑ mitochondrial number and function.
• Result: Improved neuronal resilience, energy metabolism, and repair.

2. DNA Repair & Genomic Stability

• NAD+ is a substrate for PARPs, enzymes that fix DNA strand breaks.
• This is crucial in aging, oxidative stress, and neurodegeneration,
  where DNA damage accumulates.

3. Sirtuins: Neuroprotective Guardians

• SIRT1 and SIRT3 are NAD+-dependent enzymes with anti-inflammatory and anti-apoptotic effects.
• They regulate BDNF, synaptic plasticity, and prevent mitochondrial damage in neurons.

4. Neuroinflammation Control

• NAD+ suppresses pro-inflammatory cytokines via SIRT1-mediated NF-κB inhibition.
• Reduces microglial activation and supports neuroimmune homeostasis.

NAD+ IN ADDICTION & DRUG RECOVERY

Addiction’s Impact on the Brain

• Substance abuse (alcohol, opioids, stimulants) disrupts:
  • Dopaminergic systems
  • Mitochondrial function
  • Redox balance
  • Neurotransmitter synthesis
  • Neuroplasticity

NAD+ Benefits in Addiction Recovery

1. Restoration of Brain Energy Metabolism

• Drugs of abuse deplete NAD+, leading to mitochondrial dysfunction and oxidative stress.
• IV NAD+ therapy replenishes NAD+ pools → enhances ATP, cognitive
  function, mood stabilization.

2. Reduction of Withdrawal Symptoms

• Anecdotal and clinical reports show that high-dose IV NAD+ reduces
  symptoms like:

   

  • Anxiety
  • Cravings
  • Depression
  • Fatigue
• Especially in alcohol, opioid, and stimulant detox protocols.

3. Neuroplasticity & Dopamine System Reset

• NAD+ promotes BDNF and SIRT1 signaling, aiding in dopamine receptor normalization and synaptic repair after substance abuse.

4. Epigenetic Reset & Cognitive Recovery

• NAD+-dependent enzymes regulate gene expression patterns that are disrupted by chronic drug use.
• May assist in rewiring behavior by supporting new neural pathways and breaking addictive loops.

EVIDENCE & CLINICAL INSIGHTS

Preclinical Evidence

• Rodent models show that NAD+ precursors (e.g., NR, NMN) protect
  against neurotoxicity, improve memory, and reduce addictive behavior.
• NAD+ elevation enhances GABA/glutamate balance—key in alcohol and
  benzo withdrawal.

Human Clinical & Anecdotal Use

• Clinics offering NAD+ IV therapy for detox (e.g., BR+ NAD, Springfield Wellness, NAD Clinic London) report:
  • Shortened detox timelines
  • Improved mental clarity
  • Reduced relapse risk

CLOSING THOUGHTS
NAD+ is a central regulator of brain energy, repair, and plasticity. Its ability to reset neural circuits, calm inflammation, and restore mitochondrial health makes it a promising tool in addiction recovery and neurorestoration.

While more robust clinical data is needed, early applications of NAD+—especially via IV protocols—are showing tangible benefits for those undergoing detox and seeking cognitive/mood recovery

POTENTIAL Clinical Applications:

1.Addiction Recovery & Detoxification

Clinical Context: Alcohol, opioids, benzodiazepines, stimulants Mechanism:

• Replenishes depleted NAD+ pools
• Restores neurotransmitter balance (especially dopamine and GABA)
• Reduces oxidative stress and withdrawal symptoms
  Benefits:
• Alleviates cravings, anxiety, fatigue
• May shorten detox time
• Helps with mental clarity and post acute withdrawal syndrome (PAWS)

2. Cognitive Decline & Neurodegeneration

Clinical Context: Alzheimer’s, Parkinson’s, mild cognitive impairment (MCI), poststroke recovery
Mechanism:

• Enhances mitochondrial function and synaptic repair
• Boosts neurotrophic factors (e.g., BDNF)
• Activates sirtuins and promotes DNA repair
  Benefits:
• Improved memory, focus, and mental energy
• Neuroprotective effects over time
• Slows progression in early neurodegenerative diseases

3. Anti-Aging & Longevity Support

Clinical Context: Integrative and preventive medicine, biohacking
Mechanism:

• Promotes cellular energy and autophagy
• Supports telomere maintenance and DNA repair
• Reduces senescent cell burden
  Benefits:
• Increased vitality, energy, and physical performance
• Better sleep, mood, skin health, and cognitive function
• May delay onset of age-related diseases

4. Chronic Fatigue Syndrome (CFS) / Long COVID / Mitochondrial Disorders

Clinical Context: ME/CFS, post-viral syndromes, fibromyalgia, long COVID
Mechanism:

• Recharges ATP production in dysfunctional mitochondria
• Reduces inflammation and oxidative stress
• Helps with brain fog and post-exertional malaise
  Benefits:
• Energy restoration
• Improved stamina and mental clarity
• Better exercise tolerance and mood

5. Mood Disorders & Anxiety

Clinical Context: Depression, anxiety, bipolar disorder (adjunctive), burnout
Mechanism:

• Supports serotonin, dopamine, and GABA synthesis
• Rebalances redox status and inflammation in the brain
• Enhances neuroplasticity and resilience
  Benefits:
• Mood stabilization
• Emotional regulation
• Cognitive uplift and mental energy

6. Athletic Recovery & Performance

Clinical Context: Elite athletes, overtraining syndrome, injury recovery
Mechanism:

• Supports muscle repair and mitochondrial function
• Reduces lactic acid buildup and enhances endurance
• Decreases post-exercise oxidative damage
  Benefits:
• Faster recovery
• Better stamina and endurance
• Reduced soreness and mental fatigue

7. Metabolic Health & Weight Loss (Emerging Use)

Clinical Context: Insulin resistance, metabolic syndrome, obesity
Mechanism:

• Activates sirtuins (especially SIRT1) → improved insulin sensitivity
• Enhances mitochondrial oxidation of fatty acids
• May modulate hypothalamic appetite circuits
  Benefits:
  Enhanced energy and fat metabolism
  Appetite control (especially with stacking)
  Better glucose regulation

Does the RealWorld Feedback live up to the “hype” – claimed benefits?

Updated 4–8- 25

• Yes!! in regards to Energy, Mitochondrial health, Neurorestoration –
  Repair NAD+ is easily in the TOP 5 all time discussion.
• NAD+ is easily in Top 5 for best reviewed compound in general.
  It has life changing potential which puts it into a truly unique class of tools!
• I will absolutely give it the Project Biohacked Stamp of Approval
• This has personally been a tool that has changed my life and helped in so many different ways.
• It’s not a one size fits all PANACEA compound but it performs extremely well for a very high percentage of people.

Mechanism of Action: How Does NAD+ Work?

WHAT IS NAD+?

Nicotinamide Adenine Dinucleotide (NAD⁺) is a pyridine nucleotide coenzyme present in all living cells. It serves as a:

• Coenzyme in redox reactions (NAD⁺ ↔ NADH)
• Substrate for signaling enzymes (e.g., sirtuins, PARPs, CD38)
• Metabolic regulator, linking nutrient availability to cellular function

DUAL ROLES: METABOLIC COENZYME + SIGNALING MOLECULE

1. Redox Reactions (Energy Metabolism)
Main role: Electron carrier in catabolic pathways
Key Pathways:

• Glycolysis: NAD⁺ accepts electrons → NADH
• TCA Cycle: NAD⁺ reduced to NADH in multiple steps
• Oxidative Phosphorylation: NADH donates electrons → ATP production
  in mitochondria

Why it matters: High NAD⁺ levels = more efficient ATP production and
mitochondrial function

2. Sirtuin Activation (Epigenetic Regulation & Stress Response)
Sirtuins (SIRT1–7) are NAD⁺-dependent deacetylases that modulate:

• Gene expression (via histone deacetylation)
• Mitochondrial biogenesis (via PGC-1α)
• Inflammation (via NF-κB suppression)
• Autophagy and apoptosis
  SIRT1/SIRT3 Activation by NAD⁺ =
  → Increased mitochondrial resilience
  → Reduced oxidative stress
  → Enhanced neuronal survival and synaptic plasticity

3. PARPs (DNA Repair Enzymes)
Poly(ADP-ribose) polymerases (PARPs) detect DNA damage and use NAD⁺ as a
substrate to:

• Add ADP-ribose polymers to DNA repair proteins
• Facilitate DNA strand break repair
  High DNA damage → Increased PARP activity → NAD⁺ depletion
  Chronic activation (e.g., in aging or neuroinflammation) = NAD⁺ drain → mitochondrial dysfunction

4. CD38/CD157 (NAD⁺-Consuming Enzymes)

These enzymes degrade NAD⁺ and are involved in:

• Calcium signaling (via cyclic ADP-ribose)
• Immune cell activation
• Age-related NAD⁺ decline

In aging and neuroinflammation, CD38 overactivation contributes to NAD⁺
depletion

5. Neurotransmitter Synthesis & Cognitive Function

NAD⁺ is a precursor in reactions involved in:

• Serotonin synthesis (via tryptophan metabolism)
• Dopamine balance and receptor sensitivity
• GABA/Glutamate homeostasis
  Also indirectly supports:
• BDNF expression via SIRT1 activation
• Neuronal energy metabolism and myelin maintenance

NAD⁺ DEPLETION & DYSFUNCTION

Causes of NAD⁺ decline:

• Aging
• Chronic inflammation
• Substance abuse
• Overactive PARPs/CD38
• Metabolic stress (e.g., insulin resistance)

Consequences:

• Mitochondrial dysfunction
• DNA damage accumulation
• Inflammatory cytokine surge
• Impaired neuroplasticity and cognitive decline
• Cellular senescence

NAD+ BIOSYNTHESIS PATHWAYS

1. Preiss–Handler Pathway (from nicotinic acid)
2. Salvage Pathway (from nicotinamide or NMN)

• Most dominant in mammalian cells
• Rate-limiting enzyme: NAMPT

3. De Novo Pathway (from tryptophan)

NAD⁺ precursors like NR, NMN, and NA feed into these pathways

NAMPT activity is crucial — stimulated by fasting, exercise, and certain
polyphenols (e.g., resveratrol)

Review of Literature: Studies on NAD+

1. Intravenous NAD⁺ Infusion and Cognitive Function
Study Overview: A randomized, controlled study investigated the impact of intravenous (IV) NAD⁺ administration on cognitive performance. ScienceDirect
Key Findings:

• Participants receiving IV NAD⁺ showed improvements in cognitive
  performance scores compared to baseline measurements. ScienceDirect
• The study suggests potential cognitive benefits of NAD⁺ infusion,
  warranting further research.

Reference: “Intravenous Administration of Nicotinamide Adenine Dinucleotide (NAD⁺) in Patients with Cognitive Dysfunction” ScienceDirect

2. Safety and Effectiveness of NAD⁺ Supplementation
Study Overview: A systematic review evaluated the safety and effectiveness of NAD⁺ and NADH supplementation across various clinical conditions.
Key Findings:

• NAD⁺ supplementation was generally well-tolerated.
• Reported benefits included decreased anxiety, reduced maximum heart rate after stress tests, and improved muscle insulin sensitivity. PubMed
• Some side effects were noted, such as muscle pain, fatigue, and
  headaches, but these were not considered serious. PubMed

Reference: “Evaluation of Safety and Effectiveness of NAD⁺ in Different Clinical Conditions” PubMed+1American Physiological Society+1

3. Pharmacokinetics of IV NAD⁺ Infusion
Study Overview: A pilot study examined changes in human plasma and urine
NAD⁺ levels during and after a continuous IV infusion. Frontiers
Key Findings:

• A 6-hour NAD⁺ infusion resulted in a significant increase (398%) in
  plasma NAD⁺ levels by the end of the infusion. Frontiers
• Elevated NAD⁺ levels persisted for at least 2 hours post-infusion.
• The study provided insights into the metabolism and excretion of NAD⁺ following IV administration.

Reference: “A Pilot Study Investigating Changes in the Human Plasma and Urine NAD⁺ Metabolome During a 6 Hour Intravenous Infusion of NAD⁺“

BEST RESEARCH Suppliers for NAD+:

Important Reminder: Peptides purchased direct online do not come with instructions, they are sold for RESEARCH PURPOSES ONLY – NOT FOR HUMAN CONSUMPTION.

They come in lyophilized powder form and need to be reconstituted at the proper ratio. To research peptides purchased direct SELF EDUCATION is a MUST.

Online VENDORs will NOT offer guidance on usage, it is a massive liability for them to do so. Suppliers may BLACKLIST individuals who ask about HUMAN USAGE.

IF you are not familiar w/ RESEARCH Peptides I DO not advise purchasing until you have proper education – guidance

For those buying in United States:

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For those buying in EU, UK, UAE:

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Important Reminder:
Peptides purchased online are sold FOR RESEARCH PURPOSES ONLY. This means:

• Purchasing direct online for research means you take 100% responsibility for knowing how to SAFELY research.
• Vendors will NOT advise you on how to research these compounds. They are sold for research purposes and it is a huge liability and legal risk for vendors to advise on usage. Certain vendors will blacklist those who email asking about human usage.
• Be Prepared: If you are unfamiliar with how to research peptides safely, I highly recommend enrolling in a peptide education course or working with a qualified coach or practitioner.
• Peptide Education course offer & other resources below if
  interested!

RESEARCH DOSING:

(Educational Purpose ONLY – Project Biohacked LLC)

(Educational Purpose ONLY – Project Biohacked LLC – Always consult your physician before making any changes to your medical treatment plan)

Dosing for NAD+ (subcutaneous injectable form) is a rabbit hole topic, there’s a WIDE RANGE of dosages and protocols.

Quick Dosage Range Explanation:

• There is NO one size fits all dosing approach!! No cookie cutter protocols despite many “experts” handing them out.
• The approach I have taught to clients over last 7 years is a very simple
  one.
• We start low – see how Individual responds THEN taper up based on their response.
• We look for the dosage where they notice the most benefits without any side effects.
• What dose and frequency works best per person will vary!!
• For clarification the “Microdose – Test Dose” is for those who tend to be VERY SENSITVE to exogenous compounds. People who may have a chronic health condition like MOLD, CIRs, Lyme, Autoimmune, MCAS.
• The more chemical sensitive someone is the lower they should test dose. Simple principle that ive found to work great for people 🙂

Dosage Range for NAD+ Subcutaneous INJ:

“Microdose” – Test Dose Range: 10mg – 25mg
Low – Moderate Dose Range: 25mg – 50mg
Moderate Dose: 50mg – 75mg
Moderate – Moderate High: 75mg – 100mg
High: 100mg – 125mg
Upper End: 125mg – 150mg+

How to QUICKLY Find the right dose at which you will notice benefits from NAD+:

Example of my personal approach – approach with clients:

• Someone has never tried NAD+ injectable form before.
• They decide to go with a lower test dose of 25mg.
• They notice NO side effects and NO benefits.
• The next day I would have individual trial a higher dose – likely 50mg
• Again if they notice no side effects and no benefits I would have them
  increase.
• We will repeat this process until we either notice benefits or run into the NORMAL side effects from NAD+
• Some people need to go as high as 100 – 125mg to FEEL the energy
  kick!!
• Some people unfortunately don’t feel good from NAD+…. this is rare
  and we don’t full understand why but if you experience ZERO benefits even at 100mg you may just be a non responder sadly.
• But the vast majority notice benefits and if it’s a good fit then it’s an amazing tool for a fast NATURAL non stimulating boost in energy!!

Setting Expectations and a note on NON RESPONDERS:

• Some people will notice a energy boost day 1 and at doses as low as
  10-25mg
• Majority of people find their sweet spot between 50mg – 100mg
• Some people will need to do a “loading protocol” to notice benefits.
• IE – run NAD+ for 2 weeks at 3-4x per and at the highest dose they
  tolerate side effects at. I prefer that this is in the 25mg – 100mg range
  but for some they MAY need to go higher if they notice nothing at all from 100mg.
• NON Responders – unfortunately there is a small percentage of people who do not do well with NAD+ and the possible reasons why are vast. Could be genetics, could be current toxin load, chemical sensitivity from immune based conditions. I wish I had the answer but it is complex.

Dose RANGE that works best for me after 4+ Years Cycling:

• General Fatigue Needing Quick Energy Boost 50 – 75mg
• ADD/ADHD Burnout – Heavy Fatigue – Poor Night Sleep 100 – 125mg
• Sleep deprived – Extreme Fatigue 150+mg

Couple Quick Notes on my personal usage:

• I have personally gone as high as 150mg – 200mg+ in a day w/ NAD+ but I very slowly tapered up to that higher dose.
• Typically 100mg – 150mg is plenty to give me a much needed energy boost. Those higher doses I use on days I am very sleep deprived and or going through a very high stress period.
• Per Dr. Matt Cook he spoke briefly about doing 200mg 1x per week for addiction recovery patients. Personally I would think 100mg 2-3x per week as needed would be more ideal but just my opinion based on my experience.

Frequency:

• My personal approach is 2-3x per week on a as needed basis and the few protocols from the professional community also seem to like this approach 🙂
• I turn to NAD+ on those days I really need some help with energy.
• Specially since it works extremely quick for myself it’s a tool I try to save for the times I really need it. Ive found this approach to keep it at
  maximum efficacy for myself.

Cycle Length:

• I like 8 – 12 weeks w/ 6 – 8 weeks “off” for just general health and or
  managing a higher stress period in life. This 8 – 12 week cycle also aligns with the few professional recommendations I have come across.
• There is NO set in stone approach on this part, it is highly individual
  based on goals and health situation.

Some individuals who struggle with chronic fatigue do opt to run longer term as they continue to try and address root cause of fatigue.

ChatGPT Protocols Overview:

– I find the following information to be generally pretty accurate and worth considering!

NAD+ Injectable Dosing Protocol
The dosing protocol for NAD+ injectable form depends on your goals (e.g., energy
boost, anti-aging, detox, mental clarity, or recovery). Here are some general
guidelines, but always consult with a qualified healthcare provider before
starting injectable NAD+ to ensure safety and optimal results.

1. Subcutaneous (SubQ) Injection Protocol

• Dosage: 50 mg to 100 mg per day.
  • Beginners often start with 50 mg and gradually increase to 100 mg as
    tolerated.
  • Split dosing (e.g., morning and evening) can help minimize potential
    side effects like nausea or fatigue.
• Frequency:
  • Daily for the first week to build levels.
  • After the first week, dosing can be adjusted to every other day or 2-3
    times per week for maintenance.

2. Intravenous (IV) Infusion Protocol

• Dosage: 250 mg to 1,000 mg per session.
  • Low-dose: 250-500 mg for general wellness or energy boost.
  • High-dose: 750-1,000 mg for more intensive support (e.g., addiction
    recovery, severe fatigue).
• Frequency:
  • Acute protocols: Daily or every other day for 5-10 sessions (e.g., for
    detox or recovery).
  • Maintenance: Once every 1-4 weeks, depending on individual needs.

3. Advanced Dosing Considerations

• Loading Phase:
  • For more profound benefits, start with daily NAD+ (SubQ or IV) for
    5-10 consecutive days.
  • This “loading phase” saturates the system and helps jumpstart
    energy and cellular repair.
• Maintenance Phase:
  • After the loading phase, most people can maintain results with 2-3
    SubQ injections or one IV infusion per month.
• Combination Therapy:
  • Some protocols combine NAD+ with vitamins (e.g., B12, B-complex)
    or antioxidants (e.g., glutathione) for enhanced benefits.

4. Best Practices for NAD+ Injection

• Injection Timing:
  • Morning or early afternoon is ideal, as NAD+ can boost energy and
    may interfere with sleep if taken too late.
• Hydration:
  • Stay well-hydrated before and after injections to reduce potential side
    effects.
• Side Effects:
  • Some people experience mild side effects like flushing, nausea, or
    fatigue, especially at higher doses. Starting low and slow can help
    mitigate this.

Key Notes

1. Start Low and Titrate Up:
Gradually increasing your dose allows your body to adjust and minimizes
side effects.

2. Monitor Response:
Everyone reacts differently. Keep track of how you feel and adjust dosing
or frequency as needed.

3. Professional Guidance:
Injectable NAD+ requires proper handling and should always be
administered or guided by a licensed practitioner.

By following these protocols and adjusting as needed, many people find NAD+
injections to be a transformative tool for energy, focus, and overall vitality.

Contraindications:

• Always speak to your medical professional and or independently
  research each compound to consider potential pros – cons and
  interactions with any medications.

Peptides that MAY Stack Well With NAD+:

Energy & Mitochondrial Function

1. MOTS-c

• • Why: Enhances mitochondrial biogenesis and glucose metabolism.
  • Stack synergy: Combines well with NAD+ to support metabolic
    health and increase ATP production.

2. Humanin

• • Why: Protects mitochondria and cells from oxidative stress and
    apoptosis.
  • Stack synergy: Amplifies NAD+’s protective cellular effects.

3. SS-31 (Elamipretide)

• • Why: Targets cardiolipin in mitochondria to enhance electron
    transport chain efficiency.
  • Stack synergy: Direct mitochondrial repair + NAD+ for energy
    optimization.

Neuroprotection & Cognitive Function
4. Selank

• • Why: Anxiolytic, cognitive-enhancing, and neuroprotective.
  • Stack synergy: Pairs well with NAD+’s brain bioenergetics boost.

5. Semax

• • Why: Improves neuroplasticity and BDNF expression.
  • Stack synergy: Supports cognitive performance, especially when
    NAD+ enhances neuronal energy.

6. Cortexin

• • Why: Neuropeptide complex with strong neuroprotective/
    regenerative effects.
  • Stack synergy: Complementary to NAD+ in brain recovery (poststroke, long COVID, etc.).

DNA Repair & Anti-Aging

7. Epitalon (Epithalamin)

• • Why: Telomerase activation, antioxidant, and anti-aging.
  • Stack synergy: Boosts longevity effects when combined with NAD+’s SIRT1/SIRT3 activation.

8. Thymosin Beta-4 (TB-500)

• • Why: Tissue repair, angiogenesis, and anti-inflammatory.
  • Stack synergy: Enhances tissue regeneration along with NAD+’s
    cellular recovery.

Metabolism & Recovery

9. Tesamorelin or CJC-1295/Ipamorelin

• • Why: Growth hormone stimulation for fat loss, recovery, and
    metabolism.
  • Stack synergy: GH + NAD+ can drive mitochondrial and metabolic
    upgrades.

10. AOD-9604

• • Why: Fat-burning peptide.
  • Stack synergy: Helps accelerate metabolic results from NAD+ therapy.

Medical Disclaimer:
The information provided about peptides is for educational purposes only and should not be considered medical advice. Peptide therapy is not approved by all regulatory agencies and may be considered experimental in some regions. Always consult a licensed healthcare professional before starting any peptide treatment to determine its safety, efficacy, and appropriateness for your specific health condition. Use of peptides should be supervised by qualified medical personnel, and individuals should be aware of potential risks, side effects, and contraindications. This content does not replace professional medical consultation or diagnosis.

Side Effects and Contraindications of NAD+:

Common Side Effects:

• Nausea and Gastrointestinal Discomfort: Particularly with higher
  doses, some individuals report nausea or stomach discomfort during or
  after NAD+ administration. IVDrips
• Headaches: Mild and transient headaches have been observed in some
  cases. AZ IV Medics
• Fatigue or Lethargy: Feelings of tiredness or lethargy post-infusion have
  been reported. IVDrips
• Injection Site Reactions: Pain, redness, swelling, or bruising at the
  injection site are possible, especially with subcutaneous injections.
  IVDrips+1Pure Drop IV+1
• Flushing or Warm Sensation: A sensation of warmth or skin flushing during the infusion process has been noted.

Contraindications:
Currently, there are no well-documented contraindications for NAD+
administration. However, due to limited research, it is advisable to exercise caution in certain populations: IVDrips

• Pregnant and Breastfeeding Women: The safety of NAD+ injections has
  not been established in these groups; therefore, they should avoid NAD+
  injection. Liquid Mobile IV
• Children: Due to a lack of safety data, NAD+ injections are not
  recommended for pediatric use. Liquid Mobile IV
• Individuals with Known Allergies to NAD+: Those with a known
  hypersensitivity to NAD+ should refrain from its use.

It’s important to note that while NAD+ therapy is gaining popularity,
comprehensive clinical evidence supporting its efficacy and safety is still
emerging. Consulting with a healthcare provider before initiating NAD+ therapy is recommended to ensure it aligns with individual health conditions and to monitor for potential adverse effects.

Current Review of Potential CANCER Risks:

NAD+ and Cancer Cell Metabolism:
Cancer cells often exhibit altered metabolism to support rapid proliferation. NAD+ is essential for glycolysis and other metabolic pathways that are upregulated in cancer cells. Elevated NAD+ levels can enhance these metabolic processes, potentially facilitating tumor growth. Consequently, targeting NAD+ metabolism has been proposed as a therapeutic strategy to disrupt cancer cell energy production and inhibit tumor progression. Scientific American+1PubMed Central+1

NAD+ in Cancer Therapy:
While NAD+ is vital for normal cellular functions, its role in cancer therapy is dualfaceted. Inhibiting NAD+ synthesis has shown promise in reducing tumor growth, as cancer cells rely heavily on NAD+ for their metabolic needs.Conversely, enhancing NAD+ levels may bolster anti-tumor immunity by improving the function of immune cells. This dual role suggests that therapeutic strategies should be carefully tailored to the specific cancer context. Nature+1jinfiniti.com+1Frontiers

Considerations for NAD+ Supplementation:
Given the complex relationship between NAD+ and cancer, individuals with a history of cancer or those at high risk should consult healthcare professionals before initiating NAD+ supplementation. The potential for NAD+ to support both normal and cancerous cell functions necessitates a personalized approach to its
use. PubMed Central

Conclusion:
NAD+ plays a multifaceted role in cancer biology, acting as both a supporter of essential cellular functions and a potential facilitator of tumor growth, depending on the context. Ongoing research is crucial to fully elucidate these relationships and to develop safe and effective therapeutic strategies involving NAD+
modulation.

Full List of Suppliers I personally VET:

If you love the content and want to support using my affiliate links it’s greatly appreciated and helps me afford the time to make high quality FREE content for the community which I love doing. 🙂

But no pressure!! Even if you just support by just giving quality feedback in the group and good vibes that’s appreciated too!! 🙂

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● Solution Peptides
(2024 1st Place in Voting for Favorite Peptide Supplier!!)
(Reta, Sema, NAD+, Peptides, Vitamins)
-Full 3rd party testing reports and great feedback from community on this supplier.
Group Discount Code: Biohacked15 (15% off)
https://solutionpeptides.net

● Limitless Life Nootropics
(2024 2nd Place in Voting for Favorite Peptide Supplier!!)
-Full 3rd party testing reports
Group Discount Code: Biohacked20 (20% OFF)
https://www.limitlesslifenootropics.com

● BioSynergen Peptides
Tirz Research Stocked
-Full 3rd party testing reports
Group Discount code: Biohacked10
https://biosynergen.com

● CosmicNootropic.com
(Cerebrolysin, Cortexin, Peptide Bioregulators)
Group Discount Code: projectbiohacked10 (10% off)
https://cosmicnootropic.com

● LVLUPhealth.com
For the BEST Oral Peptide Formulations – GI Healing – BPC157
Group Discount Code: Biohacked10 (10% off)
https://lvluphealth.com/en-us

● DN Lab Research is fully operational for our UK/EU and UAE Members!!
-Full 3rd party testing reports
Discount Code: Biohacked15
https://dnlabresearch.com

● Promethean Bioregulators
US Based Peptide Bioregulator supplier:
Group Discount Code: Biohacked10 (10% off)
https://www.probr.com

ATOMIK LABZ & Solution Peptides Ordering LEGEND:

• On both sites GLPs are under these different names for cautionary reasons
• ATOMIK is Solutions “sister” company separated to offer a very particular
  pep….

ATOMIK LABZ
Only group vetted source for Tirz right now*
Group Discount Code: Biohacked15 (15% off)
https://atomiklabz.com

Solution Peptides
(2024 1st Place in Voting for Favorite Peptide Supplier!!)
(Reta, Sema, NAD+, Peptides, Vitamins)
– Full 3rd party testing reports and great feedback from community on this supplier.
Group Discount Code: Biohacked15 (15% off)
https://solutionpeptides.net